Hoechst alternatives.

From: Flow Cytometry Lab (Flow.Cytometry@ed.ac.uk)
Date: Tue May 30 2000 - 09:31:05 EST


Greetings,

I am writing on behalf of an investigator who wants to assess RNA in
different populations of mouse hepatocytes using a quantitative DNA stain.
The latter must not affect the RNA so Hoechst 33342 seemed the obvious
choice. After numerous permutations of the Hoechst staining protocol (varied
concentrations, staining times and temps, addition of Verapamil )  2N, 4N
and 8N populations could not be discriminated.
If anyone has successfully stained these cells using Hoechst, or if someone
can suggest a suitable alternative, we'd appreciate your comments.

Thanks in advance,
Cathy

*****************************************************
Cathy Simpson
Flow Cytometry Laboratory
Medical School
Edinburgh University.



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