Dear All, Recently I saw that someone had asked about how to 'rescue' a valuable but infected cell line. Well, dummy me, I knew I should have saved the 'rescue' protocol for a just in case. And now, a colleague of mine (no, really, it's not me) has such a problem. Below is his comments on what he has done so far. Would the kind person who responded before please send me that 'rescue' protocol and if anyone has a recommendation for my 'colleague' I would be grateful. We are using the mouse pro-B cell line, 300.19, transfected with human ICAM-1. A clone A2 of these cells was utilized for adhesion studies. Cells were getting severely infected with somthing after 3-4 days of culture. Organisms were particulate, round to oblong in shape. They are as small or may be slightly bigger than bacteria. My cell culture contained Cipro 10microgram/ml and 1% pen-strep. It is not mycoplasma, since it can grow independent of cell. We have send it to microbiological lab and it is negative for bacteria. Used Nystatin, still could not rescue them. Any suggestion? -arup thanks in advance, Bob Bowden, PhD Laboratory Director Department of Clinical Investigations Brooke Army Medical Center, Ft Sam Houston, TX
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