At 09:21 18/05/00 -0400, you wrote: > >Hello All, > >I am in need of beads to use for absolute counts of cells which are stained with >a very bright fluorescein analog. Does anyone have experience with either >TruCounts or Flow Count beads with FITC-stained cells? Have you had problems >with compensation between The Fitc and Pe channels? Any comments would be >appreciated. Thanks > >Susan Dawes >Procter & Gamble >Miami Valley Labs >dawes.sm@pg.com > > > > Greetings Susan, I have used a bead based system for the enumeration of cells, in the system I use (see the archive for this thread), there are no compensation problems at all because; i) The beads have markedly different scatter characteristics than the cells to be counted (dendritic cells) so they may be excluded from cell phenotype analysis. ii) Where I have used this system with cell type whose scatter overlaps the bead population, there is generally no problems as the bead fluorescence is several logs more intense than the cell fluorescence and is consequently well seperated from the distrubution of interest. best regards Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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