Matthew, There are different ways to permeabolize.....formaldehyde (or dissolved paraformaldehyde-same thing), triton-X, or saponin. some commercial preps combine some of these ingredients. You might want to play with some of the times to, and temperatures during the perm stage.....if you have the time to do so, and no one has a specific answer. Good Luck! P. Echeagaray >From: Matthew Morrow <mmorrow@nih.gov> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: cytoplasmic staining II >Date: Fri, 19 May 2000 08:56:01 -0400 > > >Thanks for all who responded to my original question. Unfortunately, >I guess I left the point of it a little vague. I am interested in a >technique for staining a protein within the cytoplasm WITHOUT >staining/permeating the nucleus. I figure it's a long shot but if >anyone has any ideas, I would appreciate it. > >Matt >Matthew Morrow, MS, MT (ASCP) >Warren Magnuson Clinical Center >National Institutes of Health >Bethesda, MD >mmorrow@nih.gov/mmorrow@mail.cc.nih.gov >(301) 496-4879 ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
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