Hi Susan, You don't need to compensate for the beads in any channel. Both FlowCOUNT and TruCOUNT are broad spectrum beads which can be detected in any FL channel as a discrete bright peak. We usually look for them in FL3. If you are doing single color FITC staining use either FL2 or FL3 for the bead and adjust the PMT to put them in the top third of your histogram. If you look at beads versus time you can easily monitor the fluidics of the system or if there are any problems during aquisition. Michael Keeney ART,FIMLS Technical Specialist Hematology/Flow Cytometry London Health Sciences Centre 800 Commissioners Road East London, Ontario Canada N6A 4G5 519 685 8300 ext 52187 fax 519 685 8360 >>> "Susan Dawes-SM" <dawes.sm@pg.com> 05/18 2:30 pm >>> Hello All, I am in need of beads to use for absolute counts of cells which are stained with a very bright fluorescein analog. Does anyone have experience with either TruCounts or Flow Count beads with FITC-stained cells? Have you had problems with compensation between The Fitc and Pe channels? Any comments would be appreciated. Thanks Susan Dawes Procter & Gamble Miami Valley Labs dawes.sm@pg.com
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:20 EST