Dear Madams and Sirs, I have problems with autofluorescence of my murine bone morrow- derived dendritic cells. I received now the FACS settings for a Beckton-Dickinson FACScan which should deal with the autofluorescence of the DCs. The settings are as following: FSC Voltage: E00 1.00 Linear SSC Voltage: 303 1.83 Linear FL1 Voltage: 457 1.00 Log FL2 Voltage: 519 1.00 Log FL3 Voltage: 732 1.00 Log Compensation: FL1 - 0.1% FL2 FL2 - 84.0% FL1 FL2 - 1.0% FL3 FL3 - 20.8% FL2 I would now like to know whether a compensation of FL2 - 84.0% FL1 is acceptable because I was told that the compensation should not be over 50%. Are there other ways to overcome the problem of autofluorescence? Best regards, Karen Wahl ************************************* Karen Wahl Respiratory Medicine Unit Rayne Laboratory University of Edinburgh Medical School Teviot Place Edinburgh EH8 9AG Tel: 0131 650 6946 Fax: 0131 650 4384 e-Mail: k.wahl@ed.ac.uk Churchill House, Room 838 7 Richmond Place Edinburgh EH8 9ST Tel: 0131 662 4448 ext.: 25838 ***************************************
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