I'm wondering if anyone has used Molecular Probes reduced biohazard viability/cytotoxicity test. I'm testing activated cells effect on a breast cancer cell line in a series of T cell:Tumor cell dilutions in which the tumor cell is greatly reduced in number. I performed a similar test previously using a green lipophillic dye (DiO18) counterstained with PI. The syto/dead red dyes of this kit have the advantage of being able to stain the cells simultaneously. My question is what color combination should I use to distinguish the tumor cells from the T cells. Syto and dead red flouresce in FL1 and FL3 but I'm wondering, if anyone has used this kit before, has there been any difficulty in compensating a PE conjugated antibody or should I consider using an APC conjugated Ab? Thanks in advance for your help.
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