I tried using the Beckman Flow-Count fluorospheres as a substitute for Tru Count tubes a couple of years ago. I was disappointed to learn that I had to use the Coulter Lysis reagents (or rather, I couldn't use the BD FACSLyse) if I were to use their beads. Was I mislead about this or am I missing something? Thanks! Abby Kelliher Flow Cytometry Lab - Mass General Hospital -----Original Message----- From: Joanne Thomas [SMTP:thomas@tritechinc.com] Sent: Wednesday, May 10, 2000 11:56 AM To: Cytometry Mailing List Subject: Re: TruCount substitute Beckman Coulter sells Flow-Count Fluorospheres which can be used for absolute counting. They are sold in 20mL vials and you have to pipet them into your tube yourself, proper pipetting technique is critical for accurate results. Joanne Thomas, M.S. Director of Operations TRITECH Field Engineering 2014 Renard Court, Suite I Annapolis, MD 21401 1-800-886-7004 (USA) 1-410-266-1522 410-266-0935 (FAX) thomas@tritechinc.com -----Original Message----- From: Akos_Szilvasi@biogen.com <Akos_Szilvasi@biogen.com> To: cyto-inbox Date: Tuesday, May 09, 2000 7:31 PM Subject: TruCount substitute > > > >Dear Flowers, > >BD does not make the TruCount tubes available without buying it with their kits >(that we do not need). Does anyone know a similar product for cell counting on a >flow cytometer? > >Thanks, > >Akos Szilvasi >Biogen, Cambridge, MA > > >
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