>Dear fellow Flowers, > >we have ordered a MoFlo Sorter and, concerning simultaneous excitation in >UV and at >488nm, whether one large 5 W Argon ion laser giving both lines or an >additional smaller >laser for the 488nm is better or is there any even better third possibility?? > >Thanks a lot in advance > >Renate and Isabel >PEI flowers Hi Isabel, I think that you should go for two-laser setup because: 1) You have total control of the output power from both lasers. If you go for a dual line mirror in a large 5W Argon ion then you can only control output power from one line. The other line is tied to it. Here you would have to use Neutral density filters to achieve the desired power outputs. 2) Having two lasers gives the machine greater flexibility in terms of emission line combinations. You could have one 5W Argon with a 5W krypton for example, to give a greater range of emission lines and therefore fluorochromes. A point here I would make when ordering the MoFlo is to ensure the second laser steering tower is fitted with wide band mirrors. The tower is usually fitted with U.V. only mirrors and this is restrictive. Good luck in your choice, Andy. Andy Riddell Flow and Imaging Lab CIMR Hills Road Cambridge UK tel: (0)1223 762597 fax: (0)1223 336900 email: ar289@cam.ac.uk
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