Dear Flowperson, Using the normal sedimentation process of isolating PBMCs through Ficoll, the final PBMCs appear to be slightly shrunken. Does anyone know whether this affects PBMC functional properties, and how best to restore normal volume? Thank you Steve ************************************************************* ************************************************************* ** Stephen L. Gaffin, Ph.D. ** ** Research Physiologist ** ** U.S. Army Research Institute for Environmental Medicine ** ** Natick MA 01760-5007 ** ** Tel. 508-233-4867 Fax 508-233-4869 ** ** email stephen.gaffin@na.amedd.army.mil ** ************************************************************* *************************************************************
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