Hello Flow-ers, I had some interesting data a while back. I was staining lymphocytes for CD3,4,8, and 20 in four Rhesus samples. All four presented double populations for lymphs, monos, and grans. I have since thawed lymphocytes from the same blood draw and restained, both before and after fixation (staining is usually done after fixation). There was no evidence of double populations. My best guess as to what caused this double population was not enough fixative. (Paraformaldehyde 1.5%). The two sets of populations have different staining properties (on one the isotype is more towards the middle of the plot than the other). I am curious to hear any explaination anyone might have to offer. Thanks for your interest, Dagna Dagna Sheerar Immunology and Virology Core Laboratory Wisconsin Regional Primate Research Center University of Wisconsin 1220 Capitol Court Building #2 Room #215 Madison, WI 53715 608 265 3796 dagnas@primate.wisc.edu
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