At 09:21 09/05/00 -0400, you wrote: > > > >Dear Flowers, > >BD does not make the TruCount tubes available without buying it with their kits >(that we do not need). Does anyone know a similar product for cell counting on a >flow cytometer? > >Thanks, > >Akos Szilvasi >Biogen, Cambridge, MA > > > > > Greetings Akos, I have been using the following method to obtain absolute counts by flow; 1) centrifuge 5ml of Beckman-Coulter Flow-check Fluorospheres 400g 10mins. 2) Discard supernatent and resuspend in 5ml fetal bovine serum 3) centrifuge as above and resuspend in 5ml fetal bovine serum 4) Count beads by haemocytometer (the hard part - I count around 1000 beads) 5) Add beads to sample and analyse by flow. regards, Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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