Dear All, I think I was not clear enough since some of you sent me questions concerning my issue. I will explain that in another words. What I want to know is whether or not I can analyze data using linear scale even when I have acquired them using log scale for fluorescence acquisition mode. In another words, can I linearize data after acquisition or this is not an acceptable toll. Actually I thought that I always need to use log scale on acquisition mode for fluorescence. Thank you for your attention, Olindo
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