In response to inquiries regarding CD115 This is available from Santa Cruz (3-4A4) as unconjugated rat antibody. Copy number is relatively low, but you can clearly separate monocytes from other cells by labelling with a good rat- specific PE-labelled second antibody. If you are unsure use a biotinylated second antibody and SA-PE. Further details can be found in J.Olweus, P. A. Thompson, and F. Lundjohansen. Granulocytic and monocytic differentiation of CD34(hi) cells is associated with distinct changes in the expression of the PU.1- regulated molecules, CD64 and macrophage-colony- stimulating factor- receptor. Blood 88:3741-3754, 1996. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ PLEASE NOTE CHANGES TO CONTACT NUMBERS AND POST CODE From: Dr Richard Darley, Ph.D. Department of Haematology (A7) University of Wales College of Medicine Heath Park Cardiff, CF14 4XN U.K. tel +44 (0)29 20 74 34 85 or +44 (0)29 20 74 23 75 Fax +44 (0)29 20 74 46 55 e-mail: darley@cf.ac.uk
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:18 EST