To the Cytometry List: I have successfully used GIBCO's Enzyme-Free Cell Dissociation Buffer for many adherent cell types with the annexin V/PI assay for apoptosis. The one cell type I was unsuccessful with is HUVEC. For these cells, since I have to trypsinize the cells for the maximum viability, I use Molecular Probe's JC-1 mitochondrial stain to measure apoptosis by mitochondrial membrane depolarization. This has worked very nicely. Beverly Barton Assistant Professor Department of Surgery New Jersey Medical School-UMDNJ Newark, NJ 07103 973-972-0662
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