Hi All, Has anyone compared the Coherent I-70c with I-90/5UV for visible light performance, particularly stability? I was running the I-90 as primary laser, 488/200mW, last week swapped the I-70 into that role. I'm not happy with the result.... I don't have equipment to measure output power jitter, but the CV of bead peaks is around 2-3 times more with the I-70 compared to the same measurement done with the I-90; the FSC is now lots murkier than before. I would expect similar results from the two lasers running in exactly the same conditions, but the Coherent engineer is not convinced there is anything wrong. Any experience from the real world would be appreciated. Thanks, Joseph. -- Joseph Webster, Flow Cytometry Facility Centenary Institute, Sydney AUSTRALIA.
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