Hi Tom, you can tried to set density plots with FSC vs FL or SSC vs FL.
Some time it will help to discriminate. Also, you can change your isotype
control antibody. There is some very bad isotype control availble in the
market, they have strong Fc affinity. As a third resource, you can use an
amplification of signal approach by using biotinilated antibodies. Recently
it was widely discuss in this board about using several layers of
biotinilated reagents. Good luck and regards, Rafael
>Hi all, does anyone know of a general reference that deals with
>unresolved populations? ie, cells with low levels of antigen on
>the cell surface so they are not resolved from staining controls.
>tia td
>
>--
>==============================================================================
> Thomas M. Delohery |Internet: t-delohery@ski.mskcc.org
> Supervisor, Flow Cytometry Core Facility | Phone: (212) 639-8729
> Memorial Sloan-Kettering Cancer Center | Fax: (917) 432-2333
> 1275 York Ave. Box 98 |
> New York, NY 10021 |
>==============================================================================
\|/
(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
______________________________________________________________________________
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