Hello Claude,
FCS Assistant (shareware) allows you to export raw data from a range
of cytometry files ( the data is scaled to 10 bit for Summit files).
It also calculates ratio and allows you to then calculate a range of
statistics for each time point in a calcium experiment (percent
responding, median, mean, mode,geometric mean, 75th,90th 95th and
user-specified centiles, standard error, CV, SD), exporting the data
as an excel-readable line graph data set.
FCSPress (30 day demo, 99 pounds/149 dollars to buy) lets you plot
these line graphs as well as a range of dot, density and histogram
plots in an intuitive page layout setup.
Both programs can be downloaded from http://www.fcspress.com
The locking software for both programs has been updated recently
(today in the case of FCS Assistant) to get around conflicts with OS9
and G3/G4 computers, if your demo period has run out and you'd like
to try either program again, please request an extension from the
http://www.fcspress.com extended demo form.
Ray
At 12:13 pm -0400 1/5/00, Claude Cantin wrote:
>I have a client who would like to use raw data counts for analysis. i.e.
>channel by channel fluorescence
>values. This especially for Ca++ mobilization, so the data required is
>Fl-ratio value at a given time.
>(The client would like more than mean values of ratios for time gated
>data).
>
>I remember seeing such tables in something similar to an Excel table,
>but don't recall how it got from
>an FCS file to Excel... or whatever it was.
>
>Software we use Cyclops Summit, CellQuest, and WinList (for Mac... I'm
>aware Winlist for
>Windows is capable of doing this, but the Mac version is not).
>
>Is there freeware / shareware available to convert / import Summit /
>Cellquest data to spreadsheet
>format... and how does one go about it?
>
>Thanks everyone.
>
>Claude
--
Ray Hicks
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