Our laboratory would like to conduct an informal survey of clinical
practices. Thank you in advance for your participation. Results will be
posted to the list. You may respond privately to vbsmith@unmc.edu
Thank you,
Victoria Boryca Smith
OB-Gyn Research
UNMC
1. If a specimen is rejected due to age post draw, what is the cut-off
time for rejection?
A) >12 hours
B) >18 hours
C) >24 hours
D) >36 hours
E) >48 hours
2. On average, how old (post-draw) is specimen when stained?
A) 0 hours --stained immediately upon receipt
B) 1-6 hours
C) 6-12 hours
D) 12-24 hours
E) >24 hours
3. Do you stain before or after lysis?
4. Which lysis method does your laboratory use?
A) FACSlyse (BDB)
B) ImmunoPrep (Coulter)
C) Immunolyse (Coulter)
D) Optilyse (Coulter)
E) Uti-Lyse (Dako)
F) Ammonium Chloride
G) Other
5. How many hours is specimen stored after staining before analysis?
A) 0 hours --analysed immediately after processing
B) 1-6 hours
C) 6-12 hours
D) 12-24 hours
E) >24 hours
6. Is specimen fixed prior to analysis? Method?
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