Actually, as we've published (Bernardo et al, J. Leuk.Biol.), naive circulating monocytes as well as those incubated for 24-96 hrs and hence monocyte-derived macrophages do not all express CD 14. Those that are CD14 positive are more responsive (e.g. with a Ca++ spike) than those which are negative for CD 14, even when all are clearly monocytes. Therefore CD14 is not a good marker for monocytes in general. Elizabeth R. Simons, Ph.D. Professor of Biochemistry Boston University School of Medicine 80 E.Concord St. Boston, MA 02118 Bruce Davis wrote: > I am surprised that everyone agreed on CD14, as CD64 would get my vote as the better > marker. > > Bruce H. Davis, M.D. > Maine Medical Center Research Institute > 125 John Roberts Rd., Suite #8 > South Portland, Maine 04106 > > 207-842-7914 > FAX: 207-761-2130 > Email: davisb@mail.mmc.org > > >>> "robert gniadecki" <rgniadecki@hotmail.com> 04/27/00 09:00AM >>> > > I would like to thank everybody who answered my question. Everybody agreed > that CD14 is the best macrophage/monocyte marker. > Thanks again, > Robert Gniadecki > Copenhagen > ________________________________________________________________________ > Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
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