Diane, Some are of the opinion that log amplification of the BrdU labeling may be too sensitive, thus revealing some low level, and possibly non-chromatin DNA, content. We've looked at this both ways, and generally find the numbers to correlate. It is true that with a direct labeling technique the signals are considerably lower than with an indirect system, so linear display of the BrdU is workable. If, however, your labeling technique gives a very bright result, you may be forced to use a log display to manage it. Again, it's probably a good idea to look both ways, and see for yourself if there are significant differences. MAK. Diane M Sharp wrote: > Dear Flow Group, > > I have seen several publications lately that show BrdU cell cycle analysis, but show > both PI and FITC channels on a linear scale. I generally look at FITC on a log scale. > Does this affect results in any way? > Thanks for any comments. > > Diane Sharp > DuPont Pharmaceuticals Company > Diane.M.Sharp@dupontpharma.com --- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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