Hi We are having some problems doing calcium flux experiments (using Fluo-3 and Fura-Red) with mouse peritoneal macrophages. Even with ionomycin not all the cells respond and those that do, respond less than we would expect. Are such cells known to be hard to load? Do they sequester the dyes into cytoplasmic compartments? We have done plenty of successful experiments with other cell types. Thanks for any insights Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352
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