Hi Robert, For Monocytes in PB you can use CD14/CD45 conbination that will allow you to discriminate monocytes as CD14 high (bright) and CD45 medium (between granulocytes and lymphocytes). This conbination is considered for many scientist as the best way to gate the three majos leucocytes populations in flow (known as leucogate). I hope it helps, Olindo
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