Dear group, I am trying to setup assays of direct fluorescent staining of RBCs for CD35 and CD55. All the primary conjugated antibodies I have tried for these antigens that are commercially available give a very dim response, especially for CD35 which is known to have low expression on RBCs. I have had good results using indirect fluorescence using a secondary anti-mouse but this would preclude multicolor analysis, hence we would like to switch to direct fluorescence. Any suggestions as to which antibodies I could use or the approach? Thank you in advance for your assistance. Jose A. Stoute -- Jose A. Stoute, MD Unit 64109, Box 401 USAMRU-Kenya APO AE 09831-4109 e-mail: stoutej@net2000ke.com Nairobi Tel 254-2-729303, Fax 254-2-714592 Kisumu Tel 254-35-22942, Fax 254-35-22903 Electronic Fax Service: 1-630-214-2008, 1-917-463-0373, 1-917-477-6048
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