in my humorous approach, I'd call it "Quenching is as quenching does" and then show a tissue slide stained with a peroxidase conjugated antibody unquenched :) Then go into the discussion of what quenching is - eliminating the activity of some enzyme by saturating it with its substrate. Then if anyone asks why it needs to be done, show them that slide again :) Better yet, "This is your tissue slide. This is your tissue slide after quenching. Any questions?" __________________________________________________ Do You Yahoo!? Send online invitations with Yahoo! Invites. http://invites.yahoo.com
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