Hi, I work on FACScan cytometer and I have a problem that is shown in attachment "Problem": dendritic cell subpopulation that I analyze is always on the right side so I can not see all subpopulation in FSC/SSC dotplot diagram. So, is there any possibility to see all subpopulation, or that is the characteristic of my device that I can not change. My next question is: if I analyze those cells in FL1/FL2, what's happen with cells that are also labeled with proper Abs, but I didn't see them on FSC/SSC dotplot (because they are too much on the right side of FSC) to gate them. By the way, My name is Bratislav Janjic and I am B.Sc. of Biology. I work in Department of Pathology, University of Pittsburgh, Pennsylvania, USA and e-mail address is: royal011@visto.com I'm still not member of your society, but I hope that I can get application form to become it. Thank's in advance. Bratislav Janjic __________________________________________________________________________ Visit http://www.visto.com/info, your free web-based communications center. Visto.com. Life on the Dot.
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