CD18 combines with CD11a (to form LFA-1, expressed mainly on lymphocytes), or CD11b (to form MAC-1, expressed mainly on macrophage with low expression on lymphocytes) or with CD11c (to form a iC3b receptor, expressed mainly on macrophage). The last two are decreased in congenital "leukocyte adhesion deficience". Therefore, low expression of CD11b in total leukocyte will be of concern, but on lymphocytes CD11b is normally expected to be much lower than CD18 (where it is paired mostly with CD11a). For details about this and other CDs visit: http://www.ncbi.nlm.nih.gov/prow/ Hope that helps. Anand Shreeram Lagoo, MD, PhD Associate Professor Department of Pathology University of Mississippi Medical Center 2500 N State Street Jackson, MS 39216 (601) 984-1530; (601)984-1531 fax; (601)478-0344 pager >>> Maciej Simm <simmmmer@yahoo.com> 04/19/00 03:37PM >>> Greetings once again, We have recently received a blood specimen and were asked to stain for CD18/11b ( I love it how doctors don't explain the condition of the patient ahead of time, so we know what to look for ) We found, that when compared to our NC's (N=300+ for this "tube" and growing) this patient had less +/+ lymphoctes (~30% vs. 12%). The expression of CD18 was not reduced - all of his leukocytes had >97%. A question came up: what is the significance of CD18+/CD11b- lymphocytes? what is the significance of a decrease in the +/+ population? We do not routinely do CD11a or CD11c - are these mutually exclusive? I.e. it's either a, b or c but never any of them together? And finally, is the ligand for CD11b soluble and may have interfered with our staning? This staining was done in whole blood (which I just found is a no-no). I would appreciate if anyone could share any thoughts on this topic. Maciej __________________________________________________ Do You Yahoo!? Send online invitations with Yahoo! Invites. http://invites.yahoo.com
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