Well, there ain't nothing like embarassing yourself in front of the masses! Re: the weird "quenching" I inquired on, I have been reminded that PERCP canNOT bleed into Fl2; therefore not only is compensation unnecessary, but the effect we observed was really in the SINGLE stain tube (and not a result of the additional Ab). When the second, increasingly diluted Ab was added- the odd +/+ population shifted back to the normal +/- [quadrant 1] position. My colleagues are retesting several donors to determine if this was a one-time, bad prep or if the particular donor has some sort of interfering factor that causes CD4-Percp stained cells to fluoresce bright Fl3/2. Embarassment aside- a fresh view is ALWAYS welcome and I cannot rave enough about the help I have received from this group! Happy Easter-from the real thing! -- Bunny PS- to those that "just had to ask"; I was named this at birth- by parents who CLEARLY did not give enough thought to a future with this moniker! :-) <<ok, maybe it was after one to many beers..>> ******************************************************* Bunny Cotleur +*+ Bunny Cotleur Cleveland Clinic Foundation *+* 2001 Lester RD Neurosciences NC30 +*+ Valley City, OH 44280 9500 Euclid Avenue *+* 330-483-4800 Cleveland, OH 44195 +*+ bunny@cotleur.com 216-444-1164 *+* cotleua@ccf.org +*+ ******************************************************* When you do something, you should burn yourself completely, like a good bonfire, leaving no trace of yourself. (Shunryu Suzuki)
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:17 EST