Re: Diagram to display fluorochrom emission overlap

From: Howard Shapiro (hms@shapirolab.com)
Date: Wed Apr 19 2000 - 22:30:35 EST


Joy Mundy writes-

>Does anybody have a good diagram they can reference or send as an
>attachement that shows the spectral emission overlap for the following
>fluorchromes;
>
>FITC, PE, ECD and PCY5

There is such a diagram, unique, as far as I know, in that it shows
emission from equal concentrations of antibodies, on p. 164 of the 3rd
Edition of Practical Flow Cytometry.  If you can't find a copy of the book
nearby, I might be able to send an equivalent diagram (Wiley has the
copyright on the one in the book).

-Howard



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