p21

From: DARZYNKIEWICZ ZBIGNIEW (DARZYNK@nymc.edu)
Date: Wed Apr 19 2000 - 15:42:23 EST

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Mike Ormerod asked about p21 measurement by flow cytometry.

Can someone give me a reference to a paper describing the flow cytometric
measurement of p21 in cells?

I have searched Medline and have been unable to find anything.

Thanks.

Michael Ormerod


I presume you mean p21WAF1 rather than p21RAS. We have measured p21WAF1 by
laser scanning cytometry using PharMingen antibody  (clone 2G12) (Deptala et
al., Int. J. Oncol., 15: 861-871, 1999).
Zbigniew Darzynkiewicz

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