** Reply Requested When Convenient ** Dear colleagues, Claudio Vallan raised a query concerning staining of bacteria with PI. In the plant world, PI is considered the fluorochrome of choice for genome size determination. However, it is important that all of the samples have exactly the same treatment. Addition of paraformaldehyde may alter the penetration of the dye into interphase nuclei. Most people try and use PI at a 'saturating' concentration so that small disturbances of the concentration have minimal effect on the amount of dye bound. We use 50 mg/l. If a sample needs diluting, we use staining buffer to maintain the PI concentration. Regards Nigel Blackhall Experimental Officer, Plant Science Division, School ogf Biological Sciences, University of Nottingham, University Park Nottingham NG7 2RD. Tel 0115 9515151 x 18501 Fax 0115 9513298
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