Is it really necessary to wash cells between incubation with a
biotinylated antibody and adding streptavidin that is linked to a
fluorochrome? That is, doesn't it work as well if the two are added
together, thereby saving a step? I suppose that I could test this
myself, however I would consider limited attempts by me to be anecdotal.
Others out there may have done actual studies of this and might even
know of tricks to make it work -- assuming that it is not as simple as I
described. That's what I'm hoping, anyway!
Kevin G. Waddick, Ph.D.
Parker Hughes Institute
2657 Patton Road
St. Paul, MN 55113
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