How dangerous is a membrane impermeable dye?? Unless it can resurrect dead epithelial cells, it should not have access to the DNA of living cells, and thus shouldn't be much of a safety hazard. We always wipe everything down with ethanol, but I've yet to see data showing membrane impermeable DNA intercalating dyes cause problems for living cells. I think the MSDS claims are based on a general fear of anything that has the word "DNA" in it. Anyone have data to contradict this?? kb -- Keith Bahjat Graduate Assistant University of Florida College of Medicine Gainesville, Florida Voice: (352) 392-4887 Fax: (352) 392-5393 e-mail: kbahjat@ufl.edu > From: Scott Tighe <stighe@zoo.uvm.edu> > Organization: UVM VCC > Date: Wed, 12 Apr 2000 16:55:18 -0400 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: PI deactivation > > > > Is anyone aware of a simple method to deal with PI. We do a lot of DNA > cell cycle analysis and I would like to have a method to clean pipet > handles, keyboard,ect... I'm hoping that reacting with some type of > agent to possibly make it safer? any help would be great. > > Scott Tighe > Flow Cytometry Core Facility > Vermont cancer Ctr > A214 Medical Alumni > Burlington, Vermont 0540
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