CSF / few cells

From: Nebe, Thomas C. (thomas.nebe@ikc.ma.uni-heidelberg.de)
Date: Thu Apr 13 2000 - 05:05:36 EST

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First work was done by Prof. Dr. T.O. Kleine in Marburg 10 yrs ago.  His
protocol is published in the book "Durchflusszytometire in der klinischen
Zelldiagnostik", Schattauer, Stuttgart.
It is a no wash no lyse method on CSF using LDS 751 to gate on nucleated
cells.  The problem of analysing few cells is discussed.  He established
normal values for CSF.
There is a working group in Germany on CSF and flow operated by Dr. Mix in
Rostock (eilhard.mix@med.uni-rostock.de).
Thomas Nebe

Dr.med. C. Thomas Nebe
Universitaetsklinikum Mannheim
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