Andrea:
We have found this phenomena to be associated with a protein in the plasma of certain patients. If you wash the cells once before staining it actually goes away. Didn't believe it myself until I tried the before and after! I don't think its associated with a pathological process as some of our normal control have showed this reversible co-expression as well. Give it a try!
Joanne
Joanne Thomas, M.S.
Director of Operations
TRITECH Field Engineering
2014 Renard Court, Suite I
Annapolis, MD 21401
1-800-886-7004 (USA)
1-410-266-1522
410-266-0935 (FAX)
thomas@tritechinc.com
-----Original Message-----
From: Andrea Illingworth <dcdsflow@mint.net>
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Date: Tuesday, April 11, 2000 4:19 PM
Subject: CD4/CD8 coexpression in pb
We have noticed occasional cases with a fair amount of CD4/CD8 coexpression in peripheral bloods (up to 10% coexpression within the lymphoid gate). Most of these cases show no CMC or morphological abnormalities and we are not sure about the clinical significance of this findings. Does anybody know a "non-malignant" explanation for this finding? QC of these antibodies looks fine.
Thanks for your help!
Andrea J. Illingworth, M.S. (ASCP)
Dahl-Chase Diagnostic Services
Flow Cytometry
333 State Street
Bangor, Maine 04401
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