Dear everybody, I am doing sorting SP cells with Hoechst 33342 from human hematopoietic cells, it's a novel way to sort human hematopoietic stem cells. I have been sticking with Dr. Goodell's protocol and got nice SP cells as I presumed. But I have some problem to confirm the SP cells. As I recommended, I used Verapamil, a G-protein blocked to stain with Hoechst. I don't know what the SP cells will be changed if Verapamil is used, in short, I don't know how to confirm human SP cells. Could you be so kind to help me? Wish to hear from you! Yours sincerely, Yalin Guo Hematology & Oncology Department University Freiburg Freiburg, Germany email: guo@mm11.ukl.uni-freiburg.de Yalin Guo Nothnagel Lab Hematology/Onkology Department University of Freiburg Hugstetter Strasse 55 D-79106 Freiburg Germany Tel: +49 761 270 7199(LAB) Fax +49 761 270 7177 Email: guo@mm11.ukl.uni-freiburg.de
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