Vital-staining

From: Renate Wenig (wenre@pei.de)
Date: Mon Apr 10 2000 - 04:20:11 EST

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My name is Renate Wenig from the Paul-Ehrlich-Institut in Langen/Germany.
I'm FACS beginner.
Is there anybody with experience in vital-staining of adherent cells for
FACS-measerments?
I want to count absolute cell-number of vital cells with FACS.
Until now I try to stain the dead cells with PI but it doesn't work:
After detaching cells from the flaks bottom with EDTA all cells are PI positiv in FACS.
Can anybody give me useful tips or instructions for vital-staining of adherent cells?
Or can anybody say me where I can read about itMy E-mail adress is: wenre@pei.de
Thank you for answering!

Next message: Jamie Brewer: "annexin V stain questions"
Previous message: David Chambers: "Re: Cytometry Companies and the Internet"
Next in thread: Steve G. Hilliard: "Re: Vital-staining"
Reply: Steve G. Hilliard: "Re: Vital-staining"
Reply: Howard Shapiro: "Re: Vital-staining"
Maybe reply: Barber, Lesley (PMC): "RE: Vital-staining"
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