You should be at least extremely careful when attempting to analyse a specific enzyme with Cell Probe reagents. In contrast to our previously published work (Biol Chem Hoppe-Seyler 373: 547-54, 1992) on the intracellular analysis of elastase with rhodamine 110 based substrates, many Cell Probe reagents are not blocked against non-specific exopeptidase activity. Also except citing the specificity of an analog non-rhodamine based substrate in a cell lysate assay, no data are given about the specificity of the reagents in an intracellular assay. This information could be easily achieved using cell-permeable and specific inhibitors. I am, however, not aware of any such data for Cell Probe reagents. Gregor Rothe > -----Original Message----- > From: Christina McCowan [mailto:c.mccowan@pgrad.unimelb.edu.au] > Sent: Friday, April 07, 2000 1:43 AM > To: Cytometry Mailing List > Subject: Re: elastase in neurtophils > > > > Virginia, > > You might like also to check out the Cell Probe reagents marketed by > Beckman Coulter. > > http://134.217.3.35/coulter/cytometry/CellProbe-Reagents/cp-index.asp > > > Christina McCowan > > > At 18:49 05-04-2000 -0500, you wrote: > > > >Virginia, > > > >I have not tried this; but, you micht consider looking at this > possibility. > >http://www.phiphilux.com/elas1.htm > >I think this is suppose to be a cell permeable substrate that changes > >fluorescent properties upon protease cleavage. > >Ty Lee > > > > > >-----Original Message----- > >From: Virginia M Litwin <virginia.litwin@bms.com> > >To: cyto-inbox > >Date: Wednesday, April 05, 2000 5:12 PM > >Subject: elastase in neurtophils > > > > > >>Does anyone know of a method to measure elastase in neutorphils? > >> > > >
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