Dear Rob We do not produce the IgE directed against DNP, but we would like to inform you of our product - purified monoclonal Human IgE (kappa). It is produced in vitro by our hybridoma HE1 in accordance with ISO9001. The original antibody producing cells were obtained from a healthy donor tested negative for HIV, HCV and Hepatitis B using US-FDA approved tests. The product is purified on Protein L columns and appears as a single band after SDS-PAGE. We supply the Human IgE in vials of 0.1 mg and 1.0 mg. We can also supply the material in bulk quantities up to 1 gram on request. We have available a limited number of 0.1 mg vials for testing, free of charge. Regards Knut J. Knut J. Egelie, M. Sc., Product Manager DIATEC.COM: http://www.diatec.com Tel: +47 22 95 86 25 Fax:+47 22 95 86 49 -----Original Message----- From: R.Wadley [mailto:s9803537@pop3.unsw.edu.au] Sent: 6. april 2000 23:33 To: cyto-inbox Subject: cell line Hi Flowers Just passing this on. Regards Rob W. I am looking for a cell line of mouse origin that produces IgE directed against DNP. Our previous hybridoma (H1E-026) was sadly lost in a liquid nitrogen mishap, and I am having trouble sourcing a replacement. any help would be great, thanks ~~~~~~~~~~~~~~~~~~********************************************~~~~~~~~~~~~~~ ~~ neralie coulston school of microbiology and immunology University of New South Wales Sydney, Australia ph 9385 1081 fax 9385 1591 ******************~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~************* *** R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM Laboratory Manager Cellular Analysis Facility School of Microbiology & Immunology UNSW, New South Wales, Australia, 2052 Ph (BH) +61 (2) 9385 3517 Ph (AH) +61 (2) 9555 1239 Fax +61 (2) 9385 1591 E-mail r.wadley@unsw.edu.au www http://www.micro.unsw.edu.au/caf.html
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