hello everybody, This is not a really flow-cytometry question, but I hope someone can answer this question too. Does anyone has experiences with the cell-line Ntera 2 clone d1? My problem is that this cells grow very slowly and when I splitt the cells they will die. I used different concentrations of trypsin or cellscrapers but everytime the cells died after these procedures. What can I do ? Thanks Kerstin ***************************************** Kerstin Beck Institut für Medizinische Mikrobiologie und Hygiene Abteilung Virologie Hermann-Herder-Strasse 11 D-79104 Freiburg Telefon : +49-(0)761-203-6646 Fax : +49-(0)761-203-6603 beckk@ukl.uni-freiburg.de *****************************************
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