We are a clinical lab and we started running FACSComp once per day, but found it to be unnecessary. We were able to document that our voltages did not change much at all from week to week, so we now run FACSComp once per week, unless 1) the instrument is cleaned, 2) it is serviced, 3) there is a noticeable problem. It is good for calibrating the instrument, but most problems I see are not diagnosed by FACSComp, and I don't recommend relying on it for that purpose. Abby Kelliher Clinical Flow Cytometry Laboratory Warren Bldg. Room 112 Massachusetts General Hospital 275 Charles St. Boston, MA 02114 617-726-8487 -----Original Message----- From: CJett [SMTP:CJett@Compucyte.com] Sent: Friday, March 31, 2000 12:49 PM To: Cytometry Mailing List Subject: RE: FACSComp QC CJ> We were originally running FACSComp at the beginning of each day...but later found our stability was good enough to go to a weekly check up.....but you have to be vigilant for voltage creep on your laser..once you get around 3000 hrs the 488nm argon can start to drop off and get noisy. Of course a clinical lab will not want to do this..once a day, keeps the FDA away >>> Tami Rosario <trosario@cellmate.cb.uga.edu> 03/29/00 12:55PM >>> I have a question for those of you who run FACSComp on the FACSCaliber for QC. Do you run this each time you start up the cytometer or only occasionally. I have been told that I should run FACSComp each day that I run samples, but the beads are fairly expensive. Any alternatives? Thanks, Tami
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