Dear Sirs, I am novice in flow cytometry, though know it for a long time. But as you know, in China, no good teacher is available for me in this field. I learned much here and thank all the scientists here to supply such knowledge. Actually I am sales rep. of flow cytometry reagent in China, and we distribut PharMingen, BD, etc. You know, scientists in China as in other developing countries, they need more help than in developed countries. As sales rep. I want to do my best to make them pleased. So I will ask many questions from them, if anyone can help, we are appreciated. Thank you again for your knowledgeable information. Question today is: 1. How to detect to activation status of platelets? The customer need to know whether his patients treated with a kind of Chinese traditional medicine have a reduced level of platelet activation. The antibodies available here are anti-human CD41a, CD41b, CD61, CD62P and PAC-1, but someone said that some clones of anti-human CD41a antibodies can not discriminate the activated and unactivated form of GPIIb/IIIa. So I thought that we need such an antibody together with FSC to gate out the platelets and analyze the platelets with other parameter. So if you are experienced in this kind of detection, please give me your suggestion. Aslo, the customer need to set a rat model, so he need anti-rat reagents too, how to choose the antibodies? 2. Does anyone know a good review or reference on the relationship of cell surface markers and tumor? 3. Two parallel experiments detceted CD3/CD16+56 and CD3/CD4/CD8, the CD3 positive percent is different in the same gated cell population, what may cause such a difference? Thank all the scientists here. Sorry for my poor English. Weiwen Shen M.S. Jingmei Biotech Shanghai
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