At 12:05 31/03/00 +0100, you wrote: > >Hi! > >We are interested in measuring cellular ROS production by the use of >H2DCFDA in flowcytometry. Company instructions indicate that the dissolved >reagent has a short stability (few days). The product is provided in 100mg >size package. Thus, on one hand it is too difficult (and overall too >harmful) to weight small quantities, and, on the other, if it is necessary >to dissolve the whole amount for each test, the cost of experiments will be >too high. >We would like to know your experience about the storage conditions of >H2DCFDA, and if anyone has tested the stability of the dissolved reagent. >Thanks . > >Bye > >Angela & Anna > >Dr. Walter Giaretti >Laboratory of Biophysics and Cytometry >National Cancer Institute (IST) >Largo Rosanna Benzi, n. 10 >16132 Genoa, Italy > >Tel. +39-10-5600969 >Fax +39-10-5600711 >E-mail giaretti@hp380.ist.unige.it Greetings Angela & Anna, I have used the above reagent (molecular probes D-399) for the measurement of granulocyte ROS in a whole blood assay. It has been my experience that the reagent is unstable once dissolved in DMSO; typically we would make it up on the day of the experiment and discard any unused. We have been using a 100mg bottle of the above on and off for a few years now- it never seems to run out and at 130K Lira it couldn't be much cheaper. Also as far as I can determine, there are no particular safety issues when handling this substance. Best regards, Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 0207-209-6234 Fax: +44 0207-209-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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