We routinely use filtered DeIonized water in our FACScan and Elite flow
cytometers. The exception, of course, is when we sort. In the past when we
were running Indo-1 Calcium measurements on an Ortho Cytofluorograf, we
would also use saline. There would be a lot of noise after the baseline and
we were adding the stimulous to the sample. Saline reduced this noise so
our calcium signals were more meaningful. We have not noticed any problems
otherwise.
Doug
At 05:09 PM 3/29/2000 EST, you wrote:
>
>Hello Flowers,
>We have found that using distilled/de-ionized water in the sheath is
noisier than
>buffer on our cytometers. After puzzling over the question of contaminants
in the
>water and after some discussion with a few gurus, we have concluded that
the noise
>is not from contaminated water but may result from light bouncing off the
refractive
>index change at the water (sheath): buffer (sample core) interface.
>
>Any thoughts on this?
>
>Alice
>Alice L. Givan
>Englert Cell Analysis Laboratory
>of the Norris Cotton Cancer Center
>Dartmouth Medical School
>Lebanon, New Hampshire NH 03756
>tel 603-650-7661
>fax 603-650-6130
>givan@dartmouth.edu
>
>
>
>
{-----------------------------------------------------------------------}
Douglas Smoot
NIDDK-Navy Transplantation Augmentation Branch
Naval Medical Research Center
AFRRI Building 46, Room 2415
8901 Wisconsin Avenue
Bethesda, MD 20889-5603
voice: 301-295-1843
fax: 301-295-6484
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:14 EST