Tami Rosario wrote: > > I have a question for those of you who run FACSComp on the FACSCaliber > for QC. Do you run this each time you start up the cytometer or only > occasionally. I have been told that I should run FACSComp each day that > I run samples, but the beads are fairly expensive. Any alternatives? Tami: Glutaraldehyde Fixed Chicken erythrocytes, get them from Sigma. They're cheap and one vial will last nearly forever. Given the use of our Calibur, I check about once every week and a half, or unless a problem is reported (clog). When I've kept them at room temp, on the bench, I find that the cv's and fluorescence intensity has actually improved after about a week. My great BD service rep simply keeps them at ambient temperature in his tool kit. Hope this helps, David =========== David L. Haviland, Ph.D., Asst. Prof. Immunology University of Texas - Houston, H.S.C. Institute of Molecular Medicine, R907 2121 W. Holcombe Blvd., Houston, TX 77030 713.500.2413-Voice//713.500.2424-FAX ----------------- If everything seems to be going so well, you have obviously overlooked something. ==========
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