Re: PMT for Cy7?

From: Tom Mc Closkey (thomasm@nshs.edu)
Date: Thu Mar 30 2000 - 12:52:10 EST


Joseph Webster wrote:
>
> We want to try out APC/Cy7 on our FACStar Plus,

> I believe the standard PMT will probably not be red-sensitive
> enough for Cy7.

	Our experience on an Elite was that a "bright" antigen like CD8 was
resolvable [though poorly] using avidin APC/CY7 with the stock PMT.
Depending on your application, you may want to try it first with your OE
PMT.

Good luck,
Tom


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Thomas W. Mc Closkey, Ph. D., Director of Flow Cytometry
North Shore University Hospital, New York University School of Medicine
Boas Marks Biomedical Research Center, 350 Community Drive
Manhasset, Long Island, New York 11030
ph:  516-562-4844 [office], 516-562-1135/4641 [lab]  fax:  516-562-2866
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