Hi all, We have a problem with fitc/apc/PI stained mouse cells and Rnase to study membrane molecules and cell cycle. Mouse cells are stained with moab's. We are using ethanol or PFA as fixation and RNAse/PI treatment 30min at 37ºC prior to facs analysis. Several times we have seen that membrane staining is affected after RNAse treatment. We highly suspect the RNAse solution because 30min. 37ºC without Rnase doesn't harm the staining. Is there anyone with comments on this issue? Berend Hooibrink FACSlab CLB Amsterdam The Netherlands B_Hooibrink@CLB.NL <mailto:B_Hooibrink@CLB.NL>
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