Dictyostelium DNA measurement

From: P S Manogaran (psmano@ccmb.ap.nic.in)
Date: Wed Mar 29 2000 - 21:03:51 EST

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Dear flow experts,

 One of our staff is interested in measuring DNA content in Dictyostelium
discodium amoebae using flow techniques. The problem  is   Dictyostelium
also  carry fairly good  amount of mitochondrial  DNA , around  30% of its
total DNA . It is obvious, therefore  measuring  total DNA   does not
relate to cell cycle alone . Can anyone suggest a solution to exclude the
contribution  from mitochondrial  DNA from the total,  before or after
DNA measurement?.

Thanks in Advance

P.S.Manogaran.

Flow  cytometry facility
Centre for Cellular and Molecular Biology
Hyderabad-500007
INDIA.

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