Cell culture: Epithelial cells or fibroblasts??

From: Juergen Ostwald (juergen.ostwald@medizin.uni-rostock.de)
Date: Thu Mar 23 2000 - 10:32:02 EST


Hallo nice flowers,
please let me in with a question on the borderline of flowcytometry-
but may be that some of you fight  a similiar struggle:
I try to establish human epithelial cells (from cancers but also from
not tumour
epithelial probes prepared during the operation of patients).
But unfortunately mostly fibroblasts are growing up and epithelial cells
disapeare.
The question is: is there anybody familiar with a technique which
prefers the growth
of epithelial cells (by changing special culture conditions?)  or
does anybody knows a good forum like this in the field of cell culture
to ask there?

Thank you very much in advance
J.Ostwald
ENT-Clinic, University of Rostock, Germany



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