how about interferon gamma receptor abnormalities? mo. <<From Anne>> <<Hello list, Although this is not a strictly flow question, I was hoping some of the clinical types might have some suggestions. We are working with a 6 month old puppy with recurrent fevers that are antibiotic responsive. His white count has ranged from 30,000 to 80,000, mostly mature neutrophils (normal high for dogs about 15,000). His blood has been cultured several times and has been negative (but he was on antibiotics at the time). We carried out the following studies: 1) Immunophenotyping for CD3, CD4, CD5, CD8, CD14, CD21 and class II (all normal) 2) Immunoglobulin quantification: IgG normal, IgA normal, IgM 7 times normal 3) NBT assay for neutrophil function: normal 4) Flow cytometry with CD11c and CD11d (to rule out the possibility of a leukocyte adhesion deficiency): normal in comparison to controls (although we don't have much experience staining with these antibodies). In addition, the dog has had a single abcess that was loaded with neuts, suggesting that they can migrate out of vessels. 5) PCR for antigen receptor rearrangements to rule out lymphoma/lymphocytic leukemia on the peripheral blood: negative. Protein and immunoelectrophoresis pending to rule out an IgM producing monoclonal gammopathy, but we don't think that he has this disease. Complement levels pending as well. Hello list, Although this is not a strictly flow question, I was hoping some of the clinical types might have some suggestions. We are working with a 6 month old puppy with recurrent fevers that are antibiotic responsive. His white count has ranged from 30,000 to 80,000, mostly mature neutrophils (normal high for dogs about 15,000). His blood has been cultured several times and has been negative (but he was on antibiotics at the time). We carried out the following studies: 1) Immunophenotyping for CD3, CD4, CD5, CD8, CD14, CD21 and class II (all normal) 2) Immunoglobulin quantification: IgG normal, IgA normal, IgM 7 times normal 3) NBT assay for neutrophil function: normal 4) Flow cytometry with CD11c and CD11d (to rule out the possibility of a leukocyte adhesion deficiency): normal in comparison to controls (although we don't have much experience staining with these antibodies). In addition, the dog has had a single abcess that was loaded with neuts, suggesting that they can migrate out of vessels. 5) PCR for antigen receptor rearrangements to rule out lymphoma/lymphocytic leukemia on the peripheral blood: negative. Protein and immunoelectrophoresis pending to rule out an IgM producing monoclonal gammopathy, but we don't think that he has this disease. Complement levels pending as well. >>
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